GuideWhat is ergothioneine?Definition, transporter, food, human evidence and open questionsGuideBenefits and researchA claim-by-claim view of the evidenceResearchHealthy agingHuman and preclinical evidence for healthy-aging questionsResearchCognitive healthTrials and observational cognition researchGuideFood sourcesMushrooms and other dietary sourcesSafetyDosageResearch doses, commercial amounts and uncertaintySafetySafety and side effectsHuman safety evidence and unknownsBuyer guideSupplementsA neutral orientation to supplement formsBuyer guideHow to chooseTesting, source, COAs and cost per mg

Verified research record · Human RCT

Estimation and Validation of an Effective Ergothioneine Dose for Improved Sleep Quality Using a Physiologically Based Pharmacokinetic Model

Okumura H et al. · Food Science & Nutrition · 2025

Population
Adults enrolled in two linked dose and validation studies
Participants
See full text
Intervention
5 or 10 mg/day in dose modeling; 8 mg/day vs placebo in validation
Duration
8–16 weeks
Topics
Human, Sleep
PMID
40475978
DOI
10.1002/fsn3.70382

Key finding

The 8 mg/day validation study reported a statistically significant improvement in subjective sleep quality versus placebo.

Important limitations

Subjective outcome; linked modeling and validation design; replication by independent groups is needed.

Editorial interpretation

Preliminary human evidence for a sleep-related effect. It does not establish an optimal general supplement dose.

Funding and conflicts

Authors included researchers affiliated with Suntory Global Innovation Center; review the full disclosure.

Inspect on PubMed ↗Open DOI ↗